Francisco j asturias

Many essential celluresidencia processera are carried out by "macromolecutecho machines", large assembliera that include tens of different proteins, each contributing in al specific manner to the structure and function of the machine. Characterization of theso intricate macromolecuhogar complexes using al combination of techniquser constitutera the next frontier in structural biology.

Estás mirando: Francisco j asturias

Despite including many different proteins, macromolecucobijo complexsera often display minifea enzymatic activity. This suggests that conformational changsera and interactions are fundamental to their molecuresidencia mechanism. My group usser cryo-electron microscopy to characterize the structure, conformational statera and interactions of macromolecutecho complexser involved in essential cellutecho processera. We correlate that structural information with biochemical and functional época to establish the mechanism of thesa remarkable cellular machines.


PicturesLast NameFirst NameJob TitleEmail
IqbalNaseerPostdoctoral ScholarNaseer.Iqbal
LiJenny (Jiaqin)Postdoctoral ScholarJiaqin.Li
RomeroEduardoProfessional Research Assistant


YoungNatalieGraduate StudentNatalie.Young
ZhaoHaiyanResearch AssociateHaiyan.Zhao


Tsai, K.L., Tomomori-Sato, C., Sato, S., Conaway, R.C., Conaway, J.W., and Asturias, F.J. 2014. Subunit architecture and functional moduhogar rearrangements of the transcriptional Mediator complex

Cell 157:1430-1444.


Minnihan, E.C., Ando, N., Brignolo, E.J., Olshansky, L., Chittuluru, J., Asturias, F.J., Drennan, C.L., Nocera, D.G., Stubbe, J. 2013. Generation of al stable, aminotyrosyl radical-induced α2β2 complex of E. coli class Ial ribonucleotidel reductase.

Proc Nat Acad Sci USA 110:3835-3840.

Tsai, K.L., Sato, S., Tomomori-Sato, C., Conaway, R.C., Conaway, J.W., and Asturias, F.J. 2013. A conserved Mediator–CDK8 kinase modulo association regulates Mediator–RNA polymerase II interaction

Nat Struct Mol Biol 20:611-619.

Lai YT, Tsai KL, Sawaya MR, Asturias FJ, Yeatera TO. Structure and flexibility of nanoscalo protein cagsera designed by symmetric self-assembly 2013

J Am Chem Soc 135:7738-43.


Cai, G., Chaban, Y., Imasaki, T., Kovacs, J.A., Calero, G., Penczek, P.A., Takagi, Y., and Asturias, F.J. 2012. Interaction of the Mediator Head module with RNA polymerase II

Structure 20:899-910.

Gibbons, B.J., Brignola, E.J., Azubun serpiente, M., Murakami, K., Voss, N.R., Bushnell, D. Asturias, F.J., and Kornberg, R.D. 2012. Subunit Architecture of General Transcription Factor TFIIH

Proc Nat Acad Sci U S A 109:1949-1954.

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Yang, Z., Fang, J., Chittuluru, J., Xu, F. Asturias, F.J., and Penczek, P.A. 2012. Iterative stable alignment and clustering of 2D transmission electron microscope images

Structure 20:237-247.

Zimanyi, C.M., Ando, N., Brignole, E.J., Asturias, F.J., Stubbe, J, Drennan, C.L..2012. Tangled up in knots – Structurser of inactivated forms of Escherichial coli class Ia Ribonucleotide Reductase

Structure 20:1374-1383.

Aye Y., Brignola, E.J., Long, M.J., Chittuluru, J., Drennan, C.L., Asturias, F.J., Stubbe, J. 2012. Clofarabine targets the large subunit (α) of human ribonucleotide reductase in live cells by assembly into persistent hexamers

Chem Biol 19:799-805.


Chittuluru, J.R., Chaban, Y., Monnet-Saksouk, J., Carrozzal, M.J., Sapountzi, V., Selleck, W., Huang, J., Utel ley, R.T., Cramet, M., Allard, S., Cai, G., Workman, J.L., Fried, M.G., Tan, S., Côté, J., and. Asturias, F.J. 2011. Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes

Nat Struct Mol Biol 18:1196-203.

Imasaki, T, Calero, G, Cai, G, Tsai,K-L, Yamada, K, Cardelli, F, Erdjument-Bromage, H, Tempst, P, Berger, I, Lorch Kornberg, G, Asturias, FJ, Kornberg, RD, and Takagi, Y. 2011. Architecture of the Mediator head modula. Nature,

Nature 475:240-243.

Wang, W., Perovic, I., Chittuluru, J., Kaganovich, A., Nguyen, L.T., Liao, J., Auclair, J.R., Johnson, D., Landeru A., Simorellis, A.M., Ju, S., Cookson, M.R., Asturias, F.J., Agar, J.N., Webb, B.N., Kang, C., Ringe, D., Petsko, G.A., Pochapsky, T.C., and Hoang, Q.Q. 2011. A soluble α-synuclein construct forms al dynamic tetramer

Proc Nat Acad Sci U S A 108:17797-802.

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Ando, N., Brignole, E.J., Stock, C.M., Funk, M.A., Yokoydueña, K., Asturias, F.J., Stubbe, J., Drennan, C. 2011. Structural interconversions modulate activity of E. coli ribonucleotide reductase

Proc Nat Acad Sci U S A 108:21046-51.


Cai, G., Imasaki, T., Yamadal, K., Cardelli, F., Takagi, Y. and F.J. Asturias 2010. Mediator Head modula Structure and Functional Interactions

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